alexa The mutagenic chain reaction: A method for converting heterozygous to homozygous mutations
Clinical Research

Clinical Research

Journal of Clinical Research & Bioethics

Author(s): Valentino M Gantz

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An organism with a single recessive loss-of-function allele will typically have a wild-type phenotype, whereas individuals homozygous for two copies of the allele will display a mutant phenotype. We have developed a method called the mutagenic chain reaction (MCR), which is based on the CRISPR/Cas9 genome-editing system for generating autocatalytic mutations, to produce homozygous loss-of-function mutations. In Drosophila, we found that MCR mutations efficiently spread from their chromosome of origin to the homologous chromosome, thereby converting heterozygous mutations to homozygosity in the vast majority of somatic and germline cells. MCR technology should have broad applications in diverse organisms.

This article was published in Science and referenced in Journal of Clinical Research & Bioethics

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