Author(s): Alexander Berezin, Alexander Kremzer, Tatyana Berezina, Yulia Martovitskaya, Olena Gromenko
Accelerating atherosclerosis in Type 2 diabetes mellitus (T2DM) patients may relate with imbalance between pattern of microparticles (MPs), which frequently involved in repair of vasculature, tissue injury, inflammation and thrombosis. The aim of the study: to investigate the pattern of circulating MPs in T2DM patients with asymptomatic atherosclerosis.
The study retrospectively evolved a total of 103 patients with T2DM (54 subjects without documented coronary atherosclerosis and 49 patients with angiographic evidence of asymptomatic coronary atherosclerosis) who were undergone a contrast-enhanced multispiral tomography angiography prior study entry and 35 healthy volunteers. To determine circulating biomarkers, blood samples were collected at baseline. MPs were labeled and characterized by flow cytometry.
There were no significant differences between healthy volunteers and T2DM patients in circulating numbers of MPs labeled as CD41a+, CD64+, CD144+, CD144+/CD31+, Annexin V+, CD144+/annexin V+, and CD144+/CD31+/ annexin V+. However, lower number of MPs with immune phenotypes CD62E+, CD105E+ and higher numbers of CD31+/annexin V+ MPs were reported in T2DM patients when compared with healthy volunteers. Therefore, we found an increased level of circulating CD41a+ MPs, CD144+/CD31+ MPs, CD31+/annexin V+ MPs, and decreased level of CD62E+ MPs in T2DM patients with asymptomatic coronary atherosclerosis in comparison with those who had no asymptomatic atherosclerosis. Using multivariate Cox regression analysis, BMI (odds ratio [OR]=1.04, P=0.001), LDL-C (OR=1.05, P=0.046), hs-CRP (OR=1.07, P=0.044), osteoprotegerin (OR=1.07, P=0.026), CD62E+ MPs (OR=1.07, P=0.001) and CD31+/annexin V+ MPs (OR=1.12, P=0.003) were determined independent predictive factors of asymptomatic atherosclerosis in T2DM patients.
circulating levels of MP originated apoptotic endothelial cell-derived were significantly increased in diabetic patients as compared with normal subjects, but level of activated endothelial cell-derived MPs was lower than in healthy volunteers. Among T2DM patients only an increased level of CD31+/annexin V+ MPs and decreased CD62E+ MPs were significantly associated with asymptomatic atherosclerosis.Journal of Data Mining in Genomics & Proteomics