alexa The potential for histological screening using a combination of rapid Raman mapping and principal component analysis.


Journal of Gastrointestinal & Digestive System

Author(s): Hutchings J, Kendall C, Smith B, Shepherd N, Barr H,

Abstract Share this page

Abstract Rapid Raman mapping was carried out on 20 microm sections of oesophageal biopsy samples. Contiguous 7 microm sections were stained with haematoxylin and eosin (H&E) with histopathology provided by an expert pathologist. The step size and acquisition times were varied and the resulting spectra, principal component (PC) score maps and loads were compared. Overall mapping times were also compared to traditional Raman point mapping. The principal component loads for each of the maps were seen to be similar despite varying the acquisition time and number of spectra. Gross biochemical information was extracted showing good correlation with the H&E sections even for short overall mapping times (30-90 minutes for a 2 mm biopsy, 0.5 s acquisition time per 25.3 microm Raman pixel). This demonstrates that low signal to noise spectral maps are sufficient for the identification of histologically relevant biochemistry using principal component analysis as long as the spectral dataset is large enough. ((c) 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim). This article was published in J Biophotonics and referenced in Journal of Gastrointestinal & Digestive System

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version