Author(s): Baek JM, Howell CR, Kenerley CM, Baek JM, Howell CR, Kenerley CM
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Abstract The role of extracellular chitinase in the biocontrol activity of Trichoderma virens was examined using genetically manipulated strains of this fungus. The T. virens strains in which the chitinase gene (cht42) was disrupted (KO) or constitutively over-expressed (COE) were constructed through genetic transformation. The resulting transformants were stable and showed patterns similar to the wild-type (WT) strain with respect to growth rate, sporulation, antibiotic production, colonization efficiency on cotton roots and growth/survival in soil. Biocontrol activity of the KO and COE strains were significantly decreased and enhanced, respectively against cotton seedling disease incited by Rhizoctonia solani when compared with the WT strain.
This article was published in Curr Genet
and referenced in Journal of Bioprocessing & Biotechniques