alexa The role of spleen in vagus nerve stimulation for treatment against septic shock in rats.
Molecular Biology

Molecular Biology

Cell & Developmental Biology

Author(s): Xue N, Liang H, Yao H, Song XM, Li JG

Abstract Share this page

Abstract OBJECTIVE: To investigate the role of spleen in vagus nerve stimulation for treatment against septic shock in rats and its underlying mechanism. METHODS: Sixty-four male Sprague-Dawley (SD) rats were randomly divided into eight groups (n = 8 in each group): sham group, model group, vagotomy group, vagus nerve stimulation group, splenectomy group, splenectomy and vagus nerve stimulation group, common celiac branch vagotomy group, and selective subdiaphragmatic ventral vagotomy group. The septic shock model was reproduced by cecal ligation and puncture (CLP). All the animals were subjected to left cervical vagus nerve isolation or vagotomy, splenectomy was done 3 days before CLP, common celiac branch vagotomy and selective subdiaphragmatic ventral vagotomy were performed after CLP. Mean arterial pressure (MAP) was continuously monitored. Blood was collected 4 hours after CLP for arterial blood gas analysis. The concentrations of tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) in plasma and spleen were measured by enzyme-linked immunosorbent assay (ELISA). The spleen mRNA expressions of TNF-α and IL-1 were determined by quantitative reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Compared with sham group, MAP continuously declined, and lactic acid accumulation and metabolic acidosis appeared in model group, and the contents of TNF-α and IL-1 in plasma and spleen, and mRNA expression of TNF-α and IL-1 in spleen were significantly increased in model group C plasma TNF-α (ng/L) 113.24 5.69 vs. 24. 69 2.56, plasma IL-1 (ng/L) 226.33±9. 12 vs. 34. 58 ± 3.45; spleen TNF-α (ng/g) 286. 1216. 66 vs. 41. 33 2. 35, spleen TNF-a mRNA 1. 12+0.08 vs. 0.22± 0. 02, spleen IL-1 (ng/g) 447. 34 ± 12. 36 vs. 42. 95 ± 2. 33, spleen IL-1 mRNA 0. 93 ± 0. 06 vs. 0. 28 ± 0. 02, all P<0. 013. Compared with model group, lowering of MAP was retarded, lactic acid value and the negative value of base excess (BE) were significantly decreased, the contents of TNF-α and IL-1 in plasma and spleen, and mRNA expression of TNF-α and IL-1 in spleen Uplasma TNF-α (ng/L) 41.00 ± 3.22, plasma IL-1 (ng/L) 63.29±2. 56; spleen TNF-α (ng/g) 74. 22-3.12, spleen TNF-α mRNA 0. 32± 0. 03, spleen IL-1 (ng/g) 81. 54- 5. 48, spleen IL-1 mRNA 0. 35+0.03] were also significantly decreased in vagus nerve stimulation group (P<0. 05 or P<0. 01). However, vagus nerve stimulation after splenectomy failed to show the similar effect as seen in the vagus nerve stimulation group. Compared with vagus nerve stimulation group, the contents of TNF-a and IL-1 in plasma and spleen, and mRNA expression of TNF-α and IL-1 in spleen Eplasma TNF-a (ng/L) 118.38±8. 52, plasma IL-1 (ng/L) 252. 23 9. 55; spleen TNF-α (ng/g) 297.88± 5.44, spleen TNF-a mRNA 0. 68+0. 04, spleen IL-1 (ng/g) 450. 26 12. 45, spleen IL-1 mRNA 0. 96±0. 063 were significantly increased in common celiac branch vagotomy group (P<0. 05 or P< 0. 01). In the selective subdiaphragmatic ventral vagotomy group similar effect with that of the vagus nerve stimulation group was found. CONCLUSION: Vagus nerve stimulation fails to protect against septic shock in rats subjected to splenectomy or common celiac branch vagotomy, indicating that the spleen may be a vital target of the cholinergic anti-inflammatory pathway which is functionally linking with the spleen via the common celiac branch of vagus nerve.
This article was published in Zhongguo Wei Zhong Bing Ji Jiu Yi Xue and referenced in Cell & Developmental Biology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version