Author(s): Dorrity F Jr, Linnoila M, Habig RL
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Abstract We describe a comprehensive gas chromatographic analysis for therapeutic concentrations of amitriptyline, nortriptyline, imipramine, desipramine, doxepin, and desmethyldoxepin in plasma, with use of a nitrogen detector. All these drugs are extracted and chromatographed under identical conditions. Each tertiary amine tricyclic is well resolved from its secondary amine metabolite on a mixed-phase column and the concentrations of both are determined simultaneously, without derivatization. The lower limit of sensitivity is 10 microgram/liter of plasma (2-ml sample). Analytical recoveries of the tertiary and secondary amines are 100 and 80\%, respectively. Between-run CV's for all of the drugs ranged between 5 and 7\%.
This article was published in Clin Chem
and referenced in Chemical Sciences Journal