Author(s): Zhang XY, Inamdar NM, Supakar PC, Wu K, Ehrlich KC, , Zhang XY, Inamdar NM, Supakar PC, Wu K, Ehrlich KC,
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Abstract MDBP, a mammalian sequence-specific DNA-binding protein, was found to recognize two sites in the major immediate-early (IE) enhancer of human cytomegalovirus. The recognition sequence for MDBP at each of these sites was localized to 14 bp by studying the effects of limited G methylation, depurination, depyrimidination, or deoxyribose modification on the ability of these sites to bind to MDBP. In addition to the two high-affinity MDBP sites in the enhancer, one low-affinity MDBP site was detected 5 bp after the transcription initiating residue of this IE transcription unit. The possible biological significance of the two enhancer MDBP sites and the downstream MDBP site is discussed.
This article was published in Virology
and referenced in Cloning & Transgenesis