alexa Toluene-degrading Antarctic Pseudomonas strains from fuel-contaminated soil.
Environmental Sciences

Environmental Sciences

Journal of Bioremediation & Biodegradation

Author(s): Farrell RL, Rhodes PL, Aislabie J

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Abstract Two psychrotolerant toluene-degrading Pseudomonas spp. were isolated from JP8 jet-fuel-contaminated soils, Scott Base, Antarctica. Isolates metabolized meta-toluate as sole carbon source at temperatures ranging from 6 to 30 degrees C. Large plasmids (>64kb) were isolated from both isolates. Sequence analysis of PCR products amplified using xylB (the gene encoding benzyl alcohol dehydrogenase) primers revealed that isolates 7/167 and 8/46 were 100\% and 92\% homologous, respectively, to the xylB gene of the meta-cleavage toluene degradative pathway encoded by the TOL plasmid (pWWO) of Pseudomonas putida mt-2. Assays of cell-free extracts of 7/167 and 8/46 demonstrated activity of catechol 2,3-dioxygenase, benzyl alcohol dehydrogenase, and benzaldehyde dehydrogenase, indicating that the isolates use the meta-cleavage pathway enzymes of toluene degradation typical of TOL type plasmids. As both isolates are able to grow at 6 degrees C ex situ it is feasible that they would be able to metabolize toluene in the Antarctic soils from where they were originally isolated.
This article was published in Biochem Biophys Res Commun and referenced in Journal of Bioremediation & Biodegradation

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