alexa Toward next generation plasma profiling via heat-induced epitope retrieval and array-based assays.
Gastroenterology

Gastroenterology

Journal of Gastrointestinal & Digestive System

Author(s): Schwenk JM, Igel U, Neiman M, Langen H, Becker C,

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Abstract There is a need for high throughput methods for screening patient samples in the quest for potential biomarkers for diagnostics and patient care. Here, we used a combination of undirected target selection, antibody suspension bead arrays, and heat-induced epitope retrieval to allow for protein profiling of human plasma in a novel and systematic manner. Several antibodies were found to reveal altered protein profiles upon epitope retrieval at elevated temperatures with limits of detection improving into lower ng/ml ranges. In a study based on prostate cancer patients, several proteins with differential profiles were discovered and subsequently validated in an independent cohort. For one of the potential biomarkers, the human carnosine dipeptidase 1 protein (CNDP1), the differences were determined to be related to the glycosylation status of the targeted protein. The study shows a path of pursuit for large scale screening of biobank repositories in a flexible and proteome-wide fashion by utilizing heat-induced epitope retrieval and using an antibody suspension bead array format.
This article was published in Mol Cell Proteomics and referenced in Journal of Gastrointestinal & Digestive System

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