alexa Toward the quantification of confrontation (Dual Culture) Test: A case study on the biological control of pythium aphanidermatum with Trichoderma Asperelloides.
Environmental Sciences

Environmental Sciences

Journal of Ecosystem & Ecography

Author(s): Pakdaman BS, Goltapeh EM, Soltani BM, Talebi AA, Nadepoor M, Kruszewska JS, Pisyk S, Sarrocco S, Vannacci G

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Trichoderma species are well-appreciated filamentous fungi applied in agriculture for biological control and biofertilization purposes. One of the primary steps to screen for potent biological control isolates and/to study the effect of gene(s) on the biological control of phytopathogenic fungi and oomycetes is to perform confrontation or dual culture tests. Despite the comprehensiveness of the test, it still suffers from the lack of a reliable methodology for the mathematical data collection and statistical analyses. Here, different aspects of data collection are critically studied and new parameters are introduced. With this method the statistical comparison of different fungal biological control isolates and their counterpart phytopathogenic fungi as well as oomycetes becomes feasible.

This article was published in J Biofertil Biopestici and referenced in Journal of Ecosystem & Ecography

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