Author(s): Fry J, Barnason A, Horsch RB, Fry J, Barnason A, Horsch RB, Fry J, Barnason A, Horsch RB, Fry J, Barnason A, Horsch RB
Abstract Share this page
Abstract A reproducible system to produce transgenic Brassica napus plants has been developed using stem segments. Stem segments from 6-7 week old plants were inoculated with an Agrobacterium tumefaciens strain containing a disarmed tumor-inducing plasmid pTiT37-SE carrying a chimeric bacterial gene encoding kanamycin resistance (pMON200). Stem explants were cocultured for 2 days before transfer to kanamycin selection medium. Shoots regenerated directly from the explant in 3-6 weeks and were excised, dipped in Rootone®, and rooted in soil. Transformation was confirmed by opine production, kanamycin resistance, and DNA blot hybridization in the primary transformants. Final proof of transformation was demonstrated by the co-transfer of opine production and kanamycin resistance to progeny in a Mendelian fashion. Over 200 transgenic Brassica napus plants have been produced using this system.
This article was published in Plant Cell Rep
and referenced in Rice Research: Open Access