Author(s): Garth L Fletcher
This review focuses on the technology currently being applied to produce transgenic fish, fish containing novel gene constructs that were experimentally introduced into their genome. A number of excellent overviews on this subject have been published in recent years (1–5). Thus rather than repeat what has already been well stated we have, where possible, attempted to examine critically the published results in order to determine the factors that have or have not been established as being important to the efficient production of stable lines of transgenic fish. We hope that this exercise will be useful to researchers currently applying, or contemplating applying, transgenic technology to fish, and that it will assist all of us in the design of experiments that will enable the production of transgenic fish to be as successful as the production of transgenic mice.