alexa Transplantation of chronic wounds with epidermal sheets derived from autologous hair follicles--the Leipzig experience.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Tissue Science & Engineering

Author(s): Renner R, Harth W, Simon JC

Abstract Share this page

Abstract BACKGROUND: An increasing number of chronic wounds in our society require strategies to improve wound healing and wound closure. One of several options is skin transplantation. In this article, we focus on the transplantation of tissue engineered autologous epidermal sheets derived from outer root sheath (ORS) cells of the patients' hair. PATIENTS AND METHODS: Out of the stem cells of the ORS of anagen hair, autologous keratinocytes are cultured ex vivo in organotypic cultures to form a multilayered epidermal equivalent (EpiDex, EuroDerm Biotech & Aesthetics, Stuttgart, Germany). These sheets are placed on the wound bed. Patients were observed twice a week in the first 2 weeks, then once weekly for 4 weeks, then every 4 weeks for up to 12 weeks after transplantation. A total of 23 patients with (n = 18) and without (n = 5) therapeutic improvement were analyzed retrospectively. We evaluated only the effect of a single transplantation in a selected ulcer per patient. Furthermore, a subgroup-analysis for responder patients with an ulcer area < 25 cm2 (n = 12) was performed. RESULTS: In the responder patients (n = 18), a total wound reduction of 23\% was observed. Patients (n = 12) with ulcer area < 25 cm2 had an improvement of 64\%. Complete wound closure in this subgroup after a single transplantation was achieved in 33 \% (n = 4) cases. CONCLUSIONS: Autologous keratinocyte transplantation with EpiDex can be performed easily and safely in patients with chronic wounds with satisfying results. Our data suggest that patients with small ulcer area < 25 cm2 might profit the most from this method. This article was published in Int Wound J and referenced in Journal of Tissue Science & Engineering

Relevant Expert PPTs

Recommended Conferences

  • 6th International Conference on Tissue Engineering and Regenerative Medicine
    August 23-24 , 2017 San Francisco, California ,USA
  • 8th International Conference on Tissue Science and Regenerative Medicine
    September 11-12, 2017 Singapore City, singapore
  • 9th Annual Conference on Stem Cell and Regenerative Medicine
    Sep 25-26, 2017 Berlin, Germany
  • 10th World Congress on Stem Cell and Biobanking
    October 23-24, 2017 Osaka, Japan

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords