Author(s): Ansari NH, Bhatnagar A, Fulep E, Khanna P, Srivastava SK
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Abstract Hyperglycemia-induced cataractogenesis has been studied in rat lenses cultured in 50 mM glucose using an inverted microscope connected with a Universal C-mount and a CCD camera. Digital images were acquired and the opacity was determined by quantitating the transmitted light. Antioxidants, butylated hydroxy toluene (BHT) and 6-hydroxy-2,5,7,8-tetramethenyl-chroman-2-carboxylic acid (Trolox) provided good protection against 50 mM glucose-induced cataractogenesis in rat lenses for upto 8 days. Sorbitol levels in the 50 mM glucose+antioxidant groups were approximately 1.5 mM fold higher than in 50 mM glucose. The results, besides further demonstrating that oxidative damage is the major mechanism of sugar-induced cataractogenesis, show that Trolox or related amphipathic compounds could be of therapeutic use in the prevention of diabetic cataracts.
This article was published in Res Commun Chem Pathol Pharmacol
and referenced in Journal of Clinical & Experimental Ophthalmology