Author(s): Craandijk J, van Krugten MV, Verweij CL, van der Velden U, Loos BG
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Abstract BACKGROUND: Genetic polymorphisms for cytokines have been proposed as potential genetic markers for destructive periodontal disease. The present aim was to investigate 4 bi-allelic polymorphisms in the TNF-alpha gene in relation to susceptibility for and severity of periodontitis. The polymorphisms were all transitions from G to A, 3 in the promoter positions: -376, -308, -238; and one in the first intron at position +489. METHODS: 90 periodontitis patients and 264 reference control subjects were genotyped and frequencies of genotypes and alleles were analyzed. Also genotype frequencies among severe and moderate periodontitis patients were explored, and the \% of teeth with 50\% bone loss and the \% of teeth without any bone loss for patients with the A allele (A+ genotype) and patients without (A- genotype) were compared. RESULTS: The distributions of genotypes and frequencies of A allele carriage rates for the 4 TNF-alpha polymorphisms were not different between patients and reference controls; Hardy-Weinberg equilibrium criteria were fulfilled. Also the distribution of A+ and A- genotypes, alone or in combinations, were not different for severe periodontitis compared to moderate periodontitis patients. None of the A+ genotypes showed a significant different bone loss pattern compared to A- genotype patients. Smoking status of the patients did not influence the results. CONCLUSIONS: Genetic polymorphisms in the TNF-alpha gene at positions -376, -308, -238 and +489 could not be identified as susceptibility or severity factors in periodontitis, irrespective of the smoking status of the patients.
This article was published in J Clin Periodontol
and referenced in Journal of Forensic Research