alexa Ultra-sensitive immunosensor for beta-amyloid (1-42) using scanning tunneling microscopy-based electrical detection.
Molecular Biology

Molecular Biology

Journal of Cytology & Histology

Author(s): Kang DY, Lee JH, Oh BK, Choi JW

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Abstract An ultra-sensitive immunosensor for beta-amyloid is crucial because beta-amyloid is an important challenging marker to detect for early diagnosis of Alzheimer's disease. In this study, a vertically configured electrical detection system was developed based on scanning tunneling microscopy (STM) to detect antigen-antibody binding events. This technique could be used to easily construct a multiple measurement system in a biochip. We utilized immunocomplexes comprised of the model protein, beta-amyloid (1-42), corresponding antibody fragments, and gold (Au) nanoparticles-antibody conjugates for an immunosensor for Alzheimer's disease. The electrical tunneling current between the STM tip and these complexes exhibited a peak-like pulse, the frequency of which depended on the density of the bound complexes on the surface. We could therefore quantitatively measure beta-amyloid (1-42) concentrations as low as 10fg/mL using periodogram analysis of the peak frequency. Since this method accurately quantified much smaller amounts of beta-amyloid (1-42) than traditional immunosensors, this system shows promise as an ultra-sensitive immunodetection method. This article was published in Biosens Bioelectron and referenced in Journal of Cytology & Histology

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