alexa Ultrastructural studies of neutral lipid localisation in Streptomyces.
Microbiology

Microbiology

Journal of Microbial & Biochemical Technology

Author(s): Packter NM, Olukoshi ER

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Abstract Triacylglycerol is accumulated by Streptomyces spp. when grown in submerged culture. Ultrastructural studies using transmission electron microscopy (TEM), staining and freeze-fracture/freeze-etch procedures, and light microscopy confirmed the accumulation of neutral lipid by S. lividans and S. coelicolor during the stationary phase and its storage within membrane-bound globular structures within the cytoplasm. These structures were of various sizes and occupied up to approximately 80\% of the total cell volume at that time. There was no evidence of such material within cells examined during the early exponential phase of growth. The globules visualised by TEM were electron-transparent since they comprised lipids containing saturated fatty acids that did not react with osmium tetroxide. The globules appeared to be bounded by a single membrane.
This article was published in Arch Microbiol and referenced in Journal of Microbial & Biochemical Technology

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