Author(s): Winiewski JR, Zougman A, Nagaraj N, Mann M
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Abstract We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.
This article was published in Nat Methods
and referenced in Journal of Chromatography & Separation Techniques