alexa UPLC MS MS assay for routine quantification of dabigatran - a direct thrombin inhibitor - in human plasma.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Delavenne X, Moracchini J, Laporte S, Mismetti P, Basset T

Abstract Share this page

Abstract This article described the development and full validation of rapid and accurate liquid chromatography method, coupled with tandem mass spectrometry detection, for quantification of dabigatran in human plasma with [(13)C(6)]-dabigatran as internal standard. Plasma pretreatment involved a single step protein precipitation with methanol. Separation was performed by ultra performance reversed-phase chromatography on an Acquity UPLC BEH C8 100 mm × 1 mm × 1.7 μm column using a gradient elution mode. The mobile phase was a mix of distilled water containing 0.1\% formic acid and methanol containing 0.1\% formic acid. Specific multiple reaction monitoring transitions were recorded in positive electrospray ionization. The method was linear over the concentration range of 2-500 μg/L. The intra- and inter-day precision values were below 11.3\% and accuracy was within 93.8\% and 108.8\% for all QC levels (5, 75 and 400 μg/L). The lower limit of quantification was 2 μg/L. Total analysis time was to 10 min including sample preparation. Copyright © 2011 Elsevier B.V. All rights reserved. This article was published in J Pharm Biomed Anal and referenced in Journal of Chromatography & Separation Techniques

Relevant Expert PPTs

Recommended Conferences

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version