Author(s): SpringRobinson C, Chandramouli V, Schumann WC, Faulhaber PF, Wang Y,
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Abstract We are developing a methodology for the noninvasive imaging of glucose transport in vivo with PET and (18)F-labeled 6-fluoro-6-deoxy-d-glucose ((18)F-6FDG), a tracer that is transported but not phosphorylated. To validate the method, we evaluated the biodistribution of (18)F-6FDG to test whether it is consistent with the known properties of glucose transport, particularly with regard to insulin stimulation of glucose transport. METHODS: Under glucose clamp conditions, rats were imaged at the baseline and under conditions of hyperinsulinemia. RESULTS: The images showed that the radioactivity concentration in skeletal muscle was higher in the presence of insulin than at the baseline. We also found evidence that the metabolism of (18)F-6FDG was negligible in several tissues. CONCLUSION: (18)F-6FDG is a valid tracer that can be used in in vivo transport studies. PET studies performed under glucose clamp conditions demonstrated that the uptake of nonphosphorylated glucose transport tracer (18)F-6FDG is sensitive to insulin stimulation.
This article was published in J Nucl Med
and referenced in Clinical & Medical Biochemistry