alexa Use of retroviral vectors to introduce and express the beta-galactosidase marker gene in cultured chicken primordial germ cells.
Nephrology

Nephrology

Journal of Nephrology & Therapeutics

Author(s): Allioli N, Thomas JL, Chebloune Y, Nigon VM, Verdier G, , Allioli N, Thomas JL, Chebloune Y, Nigon VM, Verdier G,

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Abstract Three methods of isolating primordial germ cells (PGCs) from gonads of 5-day-old chick embryos were compared. PGCs were then cultured in vitro in DMEM/F12 medium containing 10\% fetal calf serum. BrdU incorporation showed that at least 10\% of the PGC population were dividing, under our culture conditions, during the 2nd day of in vitro culture. During this culture period, PGCs were exposed to avian leukosis sarcoma virus-based retroviral vector pseudotyped with subgroup A envelope, carrying the LacZ reporter gene. X-Gal staining showed that PGCs were permissive to infection, with more than 50\% of PGCs expressing the beta-Gal protein. These data represent the first demonstration that PGCs, isolated from gonads of 5-day-old chick embryos, are able to divide in vitro and that it is possible to introduce and express exogenous DNA in chick PGCs maintained in vitro. This article was published in Dev Biol and referenced in Journal of Nephrology & Therapeutics

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