alexa Usefulness of RT-PCR for the diagnosis of Japanese encephalitis in clinical samples.
Infectious Diseases

Infectious Diseases

Journal of Infectious Diseases & Therapy

Author(s): Swami R, Ratho RK, Mishra B, Singh MP

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Abstract The present study was carried out between July 2003 and December 2005 in PGIMER, Chandigarh, India and aimed to compare IgM capture ELISA and nested RT-PCR for the diagnosis of Japanese encephalitis (JE). The samples collected were cerebrospinal fluid and blood from 40 febrile patients with encephalitis (n=40, group I) and blood samples from febrile patients without encephalitis residing in JE endemic areas (n=45, group II). Overall, in CSF samples JE specific RNA was detected in 9/40 (22.5\%), while 7/28 (25\%) patients showed the presence of specific IgM antibodies. Only 28 CSF samples could be subjected to both RT-PCR and IgM and, among these, 13 cases were found to be confirmed JE based on IgM and/or RT-PCR positivity. Among the confirmed cases, 6 (6/13, 46.5\%) could be detected by RT-PCR alone, 4 (4/13, 30.7\%) by IgM capture ELISA and 3 (3/13, 23.1\%) patients were positive by both the methods. All the RT-PCR positive cases had presented within 5 d of onset of illness. The serum samples of only 16 patients in group I could be tested for IgM antibodies and 5 (31.25\%) were found to be positive, while in group II, 11.1\% (5/45) positivity was observed. JE specific RNA could not be detected in serum samples of either group of patients. This study highlights the need for carrying out RT-PCR in CSF samples, compared to IgM antibody detection, for the early detection of JEV. This article was published in Scand J Infect Dis and referenced in Journal of Infectious Diseases & Therapy

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