alexa Utilization of sweetwater as a cost-effective carbon source for sophorolipids production by Starmerella bombicola (ATCC 22214).
Chemical Engineering

Chemical Engineering

Journal of Bioprocessing & Biotechniques

Author(s): Wadekar SD, Kale SB, Lali AM, Bhowmick DN, Pratap AP, Wadekar SD, Kale SB, Lali AM, Bhowmick DN, Pratap AP

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Abstract Biosurfactants are microbially synthesized surfactants that are environmental friendly due to low toxicity. Sophorolipid is one of the simplest biosurfactants with well-defined structure produced by Starmerella bombicola(ATCC 22214) on glucose and vegetable oil as the carbon source. The raw material cost accounts for 10-30\% of the overall cost. Glycerol is readily available from a commercial fat-splitting process as sweetwater at a very low cost. Sophorolipids was synthesized using glycerol and sweetwater as a cost-effective carbon source. The glycerol was further replaced with sweetwater as a source of glycerol. Optimum glycerol concentration was 15\% w/v with 10\% w/v sunflower oil, giving 6.6 g/L of sophorolipids. The crude sophorolipid contains two major components; both of them were lactonic sophorolipids as analyzed by reverse-phase high-performance liquid chromatography (RP-HPLC), liquid chromatography-mass spectroscopy (LC-MS), and nuclear magnetic resonance ((1)H-NMR). This article was published in Prep Biochem Biotechnol and referenced in Journal of Bioprocessing & Biotechniques

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