alexa Validated HPLC method for determination of amlodipine in human plasma and its application to pharmacokinetic studies.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Zarghi A, Foroutan SM, Shafaati A, Khoddam A

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Abstract A rapid, simple and sensitive high-performance liquid chromatography (HPLC) method has been developed for quantification of amlodipine in plasma. The assay enables the measurement of amlodipine for therapeutic drug monitoring with a minimum detectable limit of 0.2 ng ml(-1). The method involves simple, one-step extraction procedure and analytical recovery was about 97\%. The separation was performed on an analytical 125 x 4.6 mm i.d. Nucleosil C8 column. The wavelength was set at 239 nm. The mobile phase was a mixture of 0.01 M sodium dihydrogen phosphate buffer and acetonitrile (63:37, v/v) adjusted to pH 3.5 at a flow rate of 1.5 ml min(-1). The calibration curve was linear over the concentration range 0.5-16 ng ml(-1). The coefficients of variation for inter-day and intra-day assay were found to be less than 10\%. This article was published in Farmaco and referenced in Journal of Chromatography & Separation Techniques

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