alexa Validation and application of chemometrics-assisted spectrophotometry and liquid chromatography for simultaneous determination of two ternary mixtures containing drotaverine hydrochloride.
Pharmaceutical Sciences

Pharmaceutical Sciences

Pharmaceutica Analytica Acta

Author(s): ElGindy A, Emara S, Shaaban H

Abstract Share this page

Abstract Three methods were developed for the determination of two ternary mixtures containing drotaverine hydrochloride (DR) with caffeine and paracetamol (mixture 1) and DR with metronidazole and diloxanide furoate (mixture 2). The first method depends on HPLC separation on an RP C18 column at ambient temperature with the mobile phase methanol-water, pH 3.1 (50 + 50, v/v) and UV detection at 213 nm for mixture 1, and the mobile phase acetonitrile-25 mM potassium dihydrogen phosphate, pH 4.6 (55 + 45, v/v) with UV detection at 235 nm for mixture 2. The other two chemometric methods applied were partial least squares (PLS-1) and principal component regression (PCR). These approaches were successfully applied to quantify the five drugs in two ternary mixtures using information included in the UV absorption spectra of appropriate solutions in the wavelength range of 210-300 nm with 1 nm intervals. Calibration of PCR and PLS-1 models was evaluated by internal validation (prediction of compounds in its own designed training set of calibration), by cross-validation (obtaining statistical parameters that show the efficiency for a calibration fit model), and by external validation (using laboratory-prepared mixtures and pharmaceutical preparations). The proposed methods were successfully applied for the determination of the two ternary combinations in laboratory-prepared mixtures and commercial tablets; the results of PLS-1 and PCR methods were compared with the HPLC method, and a good agreement was found.
This article was published in J AOAC Int and referenced in Pharmaceutica Analytica Acta

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords