alexa Validation of 24-hour fluconazole MIC readings versus the CLSI 48-hour broth microdilution reference method: results from a global Candida antifungal surveillance program.
Infectious Diseases

Infectious Diseases

Journal of AIDS & Clinical Research

Author(s): Pfaller MA, Boyken LB, Hollis RJ, Kroeger J, Messer SA, , Pfaller MA, Boyken LB, Hollis RJ, Kroeger J, Messer SA,

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Abstract We performed 24- and 48-h MIC determinations and disk diffusion testing of fluconazole against more than 11,000 clinical isolates of Candida species. By using the reference MIC breakpoints, the categorical agreement between the 24-h and reference 48-h broth microdilution results ranged from 93.8\% (all Candida species) to 94.9\% (all Candida species minus Candida krusei), with only 0.1\% very major errors (VME). The essential agreement (within 2 log(2) dilutions) between the 24-h and 48-h results was 99.6\%. The categorical agreement between the 24-h disk diffusion results and the 24-h MIC results, using the previously established breakpoints, was 94.4\%, with 0.1\% VME. Both the MIC and the disk diffusion results obtained for fluconazole after only 24 h of incubation may be used to determine the susceptibilities of Candida spp. to this widely used antifungal agent.
This article was published in J Clin Microbiol and referenced in Journal of AIDS & Clinical Research

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