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Research Article Open Access
The aim of this investigation was to go for a quantitative estimation of the caffeine content in sufficiently mature tea leaves from some tea gardens of Sonitpur district of Assam by Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) and to compare the result with that of the caffeine content of processed tea marketed in the region. The experimental findings confirmed that the caffeine content decreases with the age of tea leaves. Analysis of data using t-test by SPSS concerning percentages of caffeine in the processed tea samples of Tezpur and Gohpur sub-divisions has shown no significant difference between the processed tea samples of the two regions at 0.05 levels. But, the overall caffeine content in the samples of processed tea and mature tea leaves was found to be highly significant at 0.05 levels.
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Author(s): Parag Moni Baruah*, Santanu Bardaloi and Sunayan Bordoloi.
Caffeine, Reverse Phase-High performance Liquid Chromatography, t-test, SPSS., Plant analysis