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Research Paper Open Access
In the present work, a rapid, reliable and validated HPTLC method, which allows the quantitative determination of gallic acid in rhizomes of Curculigo orchioidesand its marketed formulation has been developed. Silica gel 60F254 plate were used as stationary phase and to1uene:ethyl acetate:glacial acetic acid (12.5:7.5:0.5 v/v) as mobile phase. The wavelength selected for analysis was 260 nm. The amount of gallic acid in the rhizome was found to be 2.54% (w/w) and in its formulation it was found to be 5.13% (w/w). The developed HPTLC method can be used as a routine method for the estimation of gallic acid in raw material and marketed formulations containing C. orchioides.
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Author(s): Spana Shrikumar M Athem M Sukumar T K Ravi