alexa Abstract | A Low-Density Protein Microarray Device for Detecting Three Antibodies Associated with Invasive Fungal Infections

Medical Mycology: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

The frequency of invasive fungal infections has risen dramatically in recent years, and early and accurate diagnosis of these infections is important for appropriate use of antifungal agents. The value of serological tests is apparent, but they suffer from disadvantages, such as being time-consuming and their inability to produce simultaneous results. Protein microarrays are a convenient technology that can be used to perform high-throughput antibody detection and enable the monitoring of different antibodies simultaneously. We constructed a low-density microarray using purified recombinant enolase (Eno) fructose-bisphosphate aldolase (Fba1) from Candida and thioredoxin reductase (TR) from Aspergillus fumigatus as immobilized antigens. Following optimization of microarray construction and serum testing, we investigated the sensitivity, repeatability, and stability of the assay using sera from patients with invasive Candida and/or Aspergillus infections and control subjects. Sera from patients with invasive candidiasis (IC) were subjected to low-density microarray assay for detection of two antibodies from Candida and one antibody from A. fumigatus. Our results indicated a sensitivity and specificity of 67.6% and 96.5% for anti-Eno and 64.8% and 90.3% for anti- Fba1 antibodies, respectively. The combined detection of anti-Eno and anti- Fba1 returned a sensitivity of 81%, and in invasive aspergillosis (IA) patients, the sensitivity and specificity of detection were 71.4% and 98.2%, respectively, for the anti-TR antibody. The low-density protein microarray assay developed in this study was capable of detecting three antibodies against two fungi simultaneously, demonstrating its potential value in diagnosing IC and IA.

 

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Xiao Chen Fangqiu Li Chunfang Ma Lining Shi and Yuan Hu

Keywords

Low-density protein microarray, Antibodies, Candida, Aspergillus, Medical Mycology, Environmental Mycology, Veterinary Mycology, Phylogeny of Fungal Pathogens, Epidemiology and Public Healt

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords