alexa Abstract | A new liquid-liquid extraction method for determination of montelukast in small volume human plasma samples using HPLC with fluorescence detector

Indian Journal of Pharmaceutical Sciences
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Montelukast is a potent orally active cysteinyl leukotriene receptor antagonist that significantly improves parameters of asthmatics. A new liquid-liquid extraction based reverse phase liquid chromatography method has been developed and subsequently validated for the determination of montelukast in human plasma. The separation was achieved with C8 column (150×4.6 mm, 5 micron) and a mobile phase comprising of a mixture of 10 mM ammonium acetate buffer (pH 3.0) and acetonitrile in a ratio of 35:65 v/v. Montelukast was extracted from human plasma using a liquid-liquid extraction technique with ter-butylmethylether. The limit of detection and lowest limit of quantification were 5 and 10 ng/ml respectively. This method was found to be linear over the range of 10 to 1000 ng/ml with a recovery of 53 to 62%. Intraday and interday precision (% CV) was <15% and accuracy ranged from 96.23 to 108.39%. Stability studies showed that montelukast in human plasma is stable during the short-term period of sample preparation and analysis. This method can be used with small volume sample during pharmacokinetic studies.

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Author(s): B Chauhan Shubha Rani M Nivsarkar H Padh

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