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Short Communication Open Access
A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the determination of rofecoxib in tablets. The analyte is resolved by using a mobile phase (methanol and water in the ratio 50:50) at a flow rate of 1 ml/min on an isocratic HPLC system (Shimadzu) consisting of LC 10AT liquid pump, SPD 10A UV-Visible detector, a ODS C-18 RP column (4.6 mm I.DX25 cm) at a wavelength of 230 nm. The linear dynamic range for rofecoxib was 2-40 Î¼g/ml by this method. Paracetamol was used as an internal standard.
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Author(s): K E V Nagoji S Vijayasrinivas M Kiran Kumar N Mathivanan M Satish Kumar M E B Rao