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Research Paper Open Access
The proposed colorimetric method for estimation of 5-Fluorouracil (5-FU) is based on the reaction between the drug and copper acetate in 3:2 chloroform : methanol mixture in presence of 0.5 percent diethylamine (DEA). The yellowish green coloured complex shows a lamda max at 350 nm and calibration curve was obtained within the range 0.4-11.0 mug/ml. The method is statistically validated and results compared favourably with those obtained by the reported method.