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Short Communication Open Access
A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica . A quantitative HPTLC method was also developed using hexane: ethyl acetate (8:2) as the mobile phase. The plumbagin content in the roots were determined using both the methods. P. indica was found to contain significantly higher amount of plumbagin than P. zeylanica . The HPLC and HPTLC methods described here are simple, rapid, accurate and sensitive.
HPLC-UV, HPTLC, Plumbago spp.