alexa Abstract | A study on some biochemical properties of active subfragment-1 (S1) prepared from actomyosin of air-breathing freshwater teleost, Channa gachua

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)


We have successfully prepared active subfragment-1 (S1) of an air-breathing teleost Channa gachua by digesting natural actomyosin (NAM) with chymotrypsin, instead of using myosin as the primary source. Conditions of preparation of acto-S1 were worked out following SDS-PAGE profiles of degradation of 200 kD MyHC to 130 kD and 94 kD products, corresponding respectively to myosin rod and S1, up to 1 hr of incubation with chymotrypsin at 15°C. The observed cleavage was accompanied by 250% activation of NAM-Ca2+-ATPase during early 30 min of digestion. Actin from acto-S1 (prepared by chymotrypsin digestion of NAM) was dissociated by Mg2+-ATP and ammonium sulfate fractionation. S1 of C. gachua prepared by this protocol had a heavy chain of 94 kD and a light chain of 19 kD. It possessed satisfactory levels of Ca2+ and K+- ATPase and Ka for dissociation of actin. The two most remarkable biochemical characteristics were : activity maxima at neutral pH and the exceptional thermostability of Ca2+ -ATPase, being the highest of any fish S1 reported so far.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Absarul Hasnain and Riaz Ahmad


Active S1, Myosin subfragment-1, Air-breathing fish, Thermostability, Natural actomyosin

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version