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Original Articles Open Access
A stability indicating High performance Liquid chromatography method was developed for the Moxifloxicin in pharmaceutical dosage forms. The chromatographic separation was achieved on C18, 150mm X 4.6mm,5 μm particle size column. The mobile phase contains a mixture of 0.1% TEA in water and methanol in isocratic elution. The retention time of Moxifloxicin was found to be 2.8min. The total run time was 10min. The proposed method is found to be having linearity in the concentration range of 20-60μg mL-1 with correlation coefficient of 0.999. The developed method has been statistically validated and found simple and accurate. The mean recoveries obtained for Moxifloxacin HCL are in the range 99.3-102%. Due to its simplicity, rapidness, high precision and accuracy of the proposed method it may be used for determining Moxifloxacin HCL in bulk and dosage forms.
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Author(s): M Vishnu Priya P Madhavan and Pramod Kumar
Method development, validation, Moxifloxicin and stability indicating., RP- HPLC