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Acrylamide (ACR) is prominent in fried, baked and heat- processed starchy foods. The present experiment was conducted to investigate the testicular toxicity of oral acrylamide exposure in male rats and the role of garlic oil in amelioration of this toxicity.
Forty-eight adult male Sprague-Dawely albino rats (weighing 120-140 g) were divided into three groups (16 rats/ group); group I: negative control group, tap water for 28 days; group II: positive control, tap water containing ACR (25 mg/kg body weight) for 28 days; and group III: tap water containing ACR (25 mg/kg body weight) and treated twice a week for 4 consecutive weeks with garlic oil (50 mg/kg body weight). Four rats were killed at 7, 14, 21 and 28 days time intervals from beginning of experiment.
The administration of ACR resulted in elevation in testes malondialdehyde (MDA) and nitric oxide (NO) levels with significant reduction in the level of glutathione (GSH) and the activity of superoxide dismutase (SOD) and catalase (CAT) in all periods of the experiment. Also, plasma testosterone was significantly elevated in ACR- treated rats as compared with the negative control. These biochemical changes were associated with congestion, interstitial edema, degeneration of spermatogenic cells in the seminiferous tubules, and formation of spermatid giant cells as well as necrosis and calcification. Treatment with garlic oil alongside ACR ameliorates this biochemical and histopathological changes.
supplementation of garlic oil, which is a sulfur containing compound act as a substrate for GSH antioxidant, may offer protection against ACR induced testicular toxicity.
Acrylamide, garlic oil, rats, testis, testosterone, oxidative stress, histopathology, Molecular Biomarkers, Biomarkers