alexa Abstract | Affinity chromatography mediated purification of hCG hormone

Journal of Chemical and Pharmaceutical Research
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Affinity chromatography by Carboxy methyl blue Sepharose was used to increase the purity and further the biological activity to more than 12000 IU/mg from 1.05mg of protein. The glycoprotein was then assessed for its conformational and functional ability in rats and could efficiently pronounce itself has a protein with increased purity

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Author(s): Uttam Kumar Neerudu S V Gopala Krishna Alamuri Shiva Kumar Aleti Amruth Rao Parveda Ramakrishna Muthineni Ramchander Merugu and Ch Venkata Ramana Devi


Affinity chromatography, CM-sepharose, biological activity, Affinity chromatography

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