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Short Communication Open Access
Two simple methods, spectrofluorimetric method and spectrophotometric method for the determination of gatifloxacin in pharmaceutical formulation are described. For spectrofluorimetric method, the excitation and emission wavelengths were found to be 365 nm and 492 nm respectively. In the case of spectrophotometric method gatifloxacin in alkaline medium on treatment with ceric ammonium sulphate and 3-methyl-2-benzothia-zolinone hydrazone gave a green colored chromogen (Î»max=452 nm). For the spectrofluorimetric and spectrophotometric methods, the linearity was found to be in the range of 0.2 to 1 Î¼g/ml and 10 to 30 Î¼g/ml respectively.
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Author(s): M Gandhimathi T K Ravi J V Susheel