alexa Abstract | Analysis of ofloxacin using UV Spectrophotometer

Journal of Prevention and Infection Control
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Research Article Open Access

Abstract

Ofloxacin belongs to the class of Floroquinolones antibiotics. This class of antibiotic is used for the treatment of both gram positive and gram negative bacterial A Simple, easy, economical and quantitative analytical method has been applied for the determination of ofloxacin using active and pharmaceutical dosage form with 2 different brands including Oflox400mg tablets manufactured by Indus Pharma Batch # 1008 and Tarivid 200mg tablet manufactured by Sanofi Aventis Batch # WD057. The maximum absorbance was detected at wavelength 294 nm using distilled water as a solvent. The linearity of the method was analyzed, ranging from 3.125ppm to 25 ppm and is found to be satisfied. Beers law was observed in the concentration range of 3.125-25 ppm with correlation coefficents 0.988, 0.998, 0.920 for standard, Oflox and Tarivid respectively which meet the criteria mentioned in the ICH guide lines (Figure 2-4). The linear regression equation obtained by least square regression method were y = 0.073x + 0.105 for active, y = 0.111x + 0.199 for Oflox and y = 0.12x + 0.527 for Tarivid which also meet the criteria mentioned in the ICH guide lines, where y is the absorbance and x is the concentration of the pure drug solution.

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Author(s): Safila Naveed and Fouzia Hamid

Keywords

Ofloxacin, Analysis ,UV, Infection Control Today

 
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