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Original Articles Open Access
The present paper describes a simple isocratic reverse phase HPLC method for the simultaneous determination and Quantification of Gestodene and Ethinyl Estradiol in Pharmaceutical dosage forms. The Seperation and estimation was achieved with normal C18(250 x4.6mm,5μ) columns using a mixture of acetonitrile,methanol and water(35:15:50) as the mobile phase at a flow rate of 2.0 ml/min and detection was carried out at 210 nm.The seperation was achieved within 18 mins and retention of Gestodene and Ethinyl Estradiol is 10.5 and 7.4 mins respectively. The developed method was validated and linearity of the proposed method was in the range of 1.88- 11.33mg/mL(r-0.9994) for Gestodene, 0.755-4.531 mg/mL (0.99973) for Ethinyl Estradiol. The proposed method was suitable for simultaneous Determination and Quantification of Gestodene and Ethinyl Estradiol in combined pharmaceutical dosage forms.
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Author(s): Kanchan Nautiyal and K Ramakrishna
Development, Validation, Tablet, Assay, HPLC, Column, Analytical