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Original Articles Open Access
The purpose of this study was to develop a simple, sensitive and validated analytical method to determine andrographolide in Andrographis paniculata. Simple Thin Layer Chromatograpy (TLC) method was performed to determine andrographolide in extracts, ethyl acetate fractions and tablets of A. paniculata with silica gel 60 F254 as stationary phase. Linear ascending development was carried out with chloroform: methanol (9:1 v/v). The Rf value of andrographolide was found to be 0.39. Camag TLC Scanner 3 was used for peak analysis and scanned at wavelenght 228 nm. The correlation coefficient of the calibration curve was 0.998 in the range of 0.08 μg to 0.60 μg. The recovery of the method was 106.74% - 109.72%. The relative standard deviation was 1.52 - 4.48%. Application this method for andrographolide determination in sample of phytopharmaceutical product development showed andrographolide content in 96% ethanol extract, 70% ethanol extract, ethyl acetate fraction-96, ethyl acetate fraction-70, ethyl acetate fraction-96 tablet and ethyl acetate fraction-70 tablet were 13.94%, 10.12%; 27.22%, 33.12%, 6.51% and 6.10%, respectively. The proposed analytical method was simple, selective and valid for determination of andrographolide in extracts, ethyl acetate fractions and tablets of A. paniculata.
TLC, andrographolide, A. paniculata, extract, ethyl acetate fraction, tablet., thin layer chromatography