alexa Abstract | Andrographolide determination of Andrographis paniculata extracts, ethyl acetate fractions and tablets by thin layer chromatography

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

The purpose of this study was to develop a simple, sensitive and validated analytical method to determine andrographolide in Andrographis paniculata. Simple Thin Layer Chromatograpy (TLC) method was performed to determine andrographolide in extracts, ethyl acetate fractions and tablets of A. paniculata with silica gel 60 F254 as stationary phase. Linear ascending development was carried out with chloroform: methanol (9:1 v/v). The Rf value of andrographolide was found to be 0.39. Camag TLC Scanner 3 was used for peak analysis and scanned at wavelenght 228 nm. The correlation coefficient of the calibration curve was 0.998 in the range of 0.08 μg to 0.60 μg. The recovery of the method was 106.74% - 109.72%. The relative standard deviation was 1.52 - 4.48%. Application this method for andrographolide determination in sample of phytopharmaceutical product development showed andrographolide content in 96% ethanol extract, 70% ethanol extract, ethyl acetate fraction-96, ethyl acetate fraction-70, ethyl acetate fraction-96 tablet and ethyl acetate fraction-70 tablet were 13.94%, 10.12%; 27.22%, 33.12%, 6.51% and 6.10%, respectively. The proposed analytical method was simple, selective and valid for determination of andrographolide in extracts, ethyl acetate fractions and tablets of A. paniculata.



Author(s): Achmad Fuad Hafid Bachtiar Rifai Lidya Tumewu Endang Widiastuti Lilis Dachliyati Riesta Primaharinastiti and Aty Widyawaruyanti

Keywords

TLC, andrographolide, A. paniculata, extract, ethyl acetate fraction, tablet., thin layer chromatography

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords