alexa Abstract | Antibacterial Activity of Hibiscus rosasinensis Extract and Synergistic Effect with Amoxicillin against some Human Pathogens

American Journal of Phytomedicine and Clinical Therapeutics
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Research Article Open Access

Abstract

Objective: Study the Antibacterial activity of Hibiscus rosa-sinensis extract and Synergistic Effect with Amoxicillin against some human pathogens.
Methods: Test microorganism for antibacterial assay (Agar well diffusion method) (Influence of combination between antibiotics and plant extracts against selected multi drug resistant strains).
Results: A total of five clinical isolates (P. aeruginosa, Serratia, Micrococcus, Enterobacter and Salmonella) belonging to different bacterial species were collected from the Department were obtained from the department of biology, college of science, Al-Mustansiryia university, Baghdad, Iraq. Co-trimoxazol, Tetracycline, Lincomycin, Enterobacter isolate was resistant to Amikacin. All bacterial isolates were sensitive to Ciprofloxacin, Norfloxacin. The minimum inhibitory concentrations (MICs) of Amoxicillin most effective antibiotics was tested against the five MDR isolates. Furthermore In the present study, only the susceptibilities of clinical bacterial isolates to the extracts of H. rosa-sinensis leaves and flowers have been examined. Therefore, further microbiological studies would be carried out to determine the minimal inhibitory concentrations (MICs) of the extracts prepared from H. rosa-sinensis against (P. aeruginosa, Serratia, Micrococcus, Enterobacter and Salmonella). The watey extracts of H. rosa-sinensis was evaluated against isolates and the results showed that the crude extracts of them with boiling water were more effective than the cold watery extracts.
Conclusion: Of this work the combination between H. rosa-sinensis and Amoxycillin showed synergistic effect against the tested bacteria than each of them alone.

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Author(s): Shaymaa Khudhr AlAlak Rasha Mohamed Sajet ALOqaili Basam Basim Mohammed and Nabaa AbdAlkhalik

Keywords

H. rosa-sinensis, P. aeruginosa, Serratia, Micrococcus, Enterobacter, Salmonella., Phytomedicine, Therapeutics

 
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