alexa Abstract | Antifungal activities of novel synthetic compounds against Phomopsis azadirachtae- the causative agent of die-back disease of neem

Journal of Chemical and Pharmaceutical Research
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Abstract

Seven novel synthetic heterocyclic compounds containing naphthofurans were evaluated for their inhibitory effect on Phomopsis azadirachtae, the causative agent of destructive die-back disease of neem. Twigs of Azadirachta indica (Neem) infected with die-back were collected and were analyzed to determine the pathogen. Phomopsis azadirachtae the causal organism was isolated on malt extract agar from die-back infected neem twigs. They were identified by PCR based molecular methods. Phomopsis genus specific primers (5.8S r-DNA) were then used for the confirmation of P. azadirachtae – the causative agent of die-back of neem by Polymerase chain reaction (PCR). Studies revealed the amplification of expected 141bp DNA in P. azadirachtae isolated from the diseased trees confirming the causal organism of die-back of neem. Studies revealed a very effective in vitro control of P.azadirachtae mycelia growth at very significant concentration.



Author(s): Nagendra Prasad MNab Vivek H Ka Ashwini Prasadb Shruthi Ta Shankara Bhat SbNagaraja G Kc and Nanjunda Swamy Say

Keywords

Phomopsis azadirachtae, Azadirachta indica, naphthofurans, die-back of neem, Polymerase chain reaction.

 
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