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Original Articles Open Access
Various plants have been used traditionally by communities for treatment cardiovascular disease. In this research, fruit extracts of Solanum Muricatum Aitonwas studied. The aim of this research was to determine the effect of antioxidative potential of these extracts on the ability to modulate apoptotic in yeast (Saccharomyces cerevisiae FNCC 3012) cells. Antioxidative potentials were analyzed using Thiobarbituric acid (TBA) assay by measuring the concentration of Malonaldehyde (MDA) upon linoleic acid oxidation using spectrophotometer UV-Vis at 532 nm and vitamin E was used as a control antioxidant. Apoptotic activity was determined by counting the colony of yeast cells and measuring the frequency of petite yeast cells after treatment with extract followed by incubation. The results of viability test showed that petit cells at glucose 4% more than 60%, whereas control of aquadest seen its amount only 35,05%. The raising level of concentration caused petit cells percentage is bigger, whereas at concentration 300 ppm it was be down. From this research, it can be concluded that Solanum Muricatum Aiton extract showed midle antioxidative potential as well as midle inhibition of apoptotic activity in yeast cells (Saccharomyces cerevisiae FNCC 3012).
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Author(s): Devi Ratnawati and Eni Widiyati
Solanum Muricatum Aiton, Antioxidant, Saccharomyces cerevisiae FNCC 3012., Antioxidant