alexa Abstract | Antioxidant activities of various fruit extracts from three solanum sp. using DPPH and ABTS method and correlation with phenolic, flavonoid and carotenoid content

Journal of Chemical and Pharmaceutical Research
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Abstract

The objectives of this research were to study antioxidant activities from various fruit extracts of three Solanum sp using two methods of antioxidant assays which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’- azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) and correlation of total flavonoid, phenolic, and carotenoid content in various fruit extracts of three Solanum sp with IC50 of DPPH antioxidant activities and IC50 of ABTS antioxidant activities. Extraction was conducted by reflux apparatus using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant capacities were tested using DPPH and ABTS assays. Determination of total phenolic, and carotenoid content performed by UV-visible and their correlation with IC50 of DPPH and IC50 of ABTS antioxidant activities were analyzed by Pearson’s method. Ethanolic fruit extract of S. torvum (ST3) had the lowest IC50 of DPPH scavenging activity 1.4 μg/ml, while ethanolic fruit extract of S. coagulans (SC3) had the lowest IC50 of ABTS scavenging activity 1.5 μg/ml. Ethanolic fruit extract of S. torvum (ST3) the highest total phenolic content (5.15 g GAE/100 g), ethyl acetate fruit extract of S. americanum (SA2) had the highest total flavonoid content (9.37 g QE/100 g), and the highest total carotenoid content (0.87 g BE/100 g). There were negatively high correlation between total phenolic content in all of fruit extracts of S. americanum, S. torvum, S. coagulans with their IC50 of DPPH and IC50 of IC50 of ABTS scavenging activities. The IC50 of DPPH scavenging activities of S. americanum, S. torvum, S. coagulans fruit extracts gave linear result with their IC50 of ABTS scavenging activities.

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Author(s): Irda Fidrianny Atina Rizkiya and Komar Ruslan

Keywords

Antioxidant, DPPH, ABTS, fruit, three Solanum sp, phenolic, flavonoid, carotenoid, Antioxidant activities,flavonoid

 
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