700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ ReadersThis Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Research Article Open Access
Two simple, accurate and sensitive analytical methods have been developed for the simultaneous determination of Rabeprazole and Domperidone in pharmaceutical solid dosage form. The first method involves UV-spectrophotometric determination of both drugs in combination, using Qanalysis method. It involves absorbance measurement at 291.6 nm (λmax of Rabeprazole) and 240 nm isobestic point in solvent (0.1N NaoH), Sodium hydroxide. The method obeys Beer’s law over linearity range for Rabeprazole 2 – 12 μg mL-1 and for Domperidone 3-18 μg mL-1 respectively. The second method involves HPLC separation of Rabeprazole and Domperidone drugs in reverse phase mode using Phenomenx C18 column. Linearity was obtained in the concentration range of 0.5-5.0 μg mL-1 for Rabeprazole and 0.75-7.5 μg mL-1 Domperidone. The detection wavelength was 220 nm. Pantaprazole was used as internal standard in10 μg mL–1 concentration. Relative standard deviation (R.S.D) for both methods was found <2.0%.The proposed methods were successively applied to muticomponent formulation containing both the drugs. The methods were validated according to ICH guidelines.
To read the full article Peer-reviewed Article PDF
Author(s): Arunadevi S Birajdar Subramania N Meyyanathan Bojraj Suresh
Rabeprazole, Domperidone, Pantaprazole, UV- spectrophotometry, RP-HPLC