alexa Abstract | Assay of riboflavin carrier protein and its APO form using UV-visible spectrophotometry

Journal of Chemical and Pharmaceutical Research
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Riboflavin carrier protein from spotted owlet egg yolk has been isolated and it was assayed using spectrophotometry. The spectral intensity (1.687) of Rf solution showed small changes with a gradually increase in Riboflavin Carrier protein Binding capacity initially 10μl to 40μl and decreased binding capacity later on between 50 to 60 μl concentration. Isolated protein (RCP) binding affinity to Riboflavin was calculated at different concentrations and the results are communicated

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Author(s): Karunakar Rao Kudle and M P Pratap Rudra


RCP = Riboflavin Carrier Protein, RF= Riboflavin, RCP-Riboflavin Complex, UV-visible spectrophotometry

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