alexa Abstract | Assessment of DNA damage of women using oral contraceptives by high resolution melt analysis

European Journal of Experimental Biology
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Research Article Open Access

Abstract

The aim of the study was to evaluate the genotoxic effect of combined oral contraceptives with low concentration of estrogens: 0.03 mg ethinylestradiol and 3 mg desogestrel per day using HRM analysis of genomic DNA isolated from the whole unstimulated saliva. Our data suggest that there are not significant alterations in the melting temperature of DNA of the group exposed to the oral contraception in comparison with the control group. The sequenation (MDR1 gene, a forward primer 5´-TGGGGCTTTTAGTGTTGGAC-3´; a backward primer 5´- TGTGGAGAGCTGGATAAAGTCA-3´) confirmed identical nucleotide sequences for forward fragments of both groups. In spite of this, minor change of nucleotide content were observed when compared the sequences of DNA for the control group and the volunteers group taking oral contraceptives for backward fragments, when a deletion in the sequence on the 50 bp position for the group exposed to the oral contraception was observed.

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Author(s): Marianna Trebuovaacute Galina Laputkovaacute and Jozef ivaacutek

Keywords

Melting analysis, DNA, Saliva, Contraception, MDR1 gene

 
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