alexa Abstract | Calcium-Activated Chloride Channel in Rat Pulmonary Artery Smooth Muscle Cells

Electronic Journal of Biology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Abstract

Calcium-activated chloride channels (CaCC) are crucial regulators of vascular tone by promoting a depolarizing influence on the resting membrane potential of vascular smooth muscle cells. The chloride equilibrium potential (ECl) of pulmonary artery smooth muscle cells (PASMC) is significantly more positive than the resting potential. The role of CaCC is probably to produce membrane depolarization and consequent opening of voltage-dependent calcium channels (VDCCs) to evoke contraction by influx of extracellular Ca2+. Using the whole cell patch-clamp technique, sustained Ca2+-activated Cl- currents (ICl(Ca)) evoked by K+ free pipette solutions containing 500nM Ca2+ were recorded in single rat pulmonary artery smooth muscle cell. The electrophysiological characters of CaCC and its possible contributions to the rat pulmonary artery tone were studied. In conclusion, ICl(Ca) exhibited the typical outwardly rectifying. The steady states currents and activation/inactivation time constant of ICl(Ca) have obvious voltage dependence when evoked by fixed 500nM [Ca2+]i. However, increasing the duration of a +70 mV test pulse from 200 to 1400 ms didn’t significantly augment the amplitude of the outward current relaxation and tail current. The possible reasons are discussed.

To read the full article Peer-reviewed Article PDF image

Author(s): Shangbang Gao Changming Wang Weiwei Yu Biwen Mo Chenhong Li

Keywords

Calcium-activated chloride channel, Pulmonary artery, Smooth muscle cells, Patch clamp, Whole cell, Electrophysiological characters.

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords