alexa Abstract | Carotenogenesis Study of Neurospora intermedia N-1 in liquid substrate fermentation

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

Neurospora intermedia N-1 is a fungus isolated from “oncom merah”, an Indonesian red peanut cake. It has been studied for carotenoids production for food and drug colorant. Commercial production of natural carotenoids from microorganisms recently competes mainly with synthetic carotenoids by chemical procedures. Improving the efficiency of carotenoid biosynthesis can increase carotenoids production by microorganisms. Some of the cultural and environmental factors are positively affect carotenoids content of the fungal strains. The objective of this study was to evaluate the carotenogenesis of Neurospora intermedia N-1 in a liquid fermentation. The fermentation was started by optimizing the condition with light responses, then addition of variated carbon sources at 2 % w/v, cofactor Mg2+, antibiotics penicillin and chloramphenicol. Carotenoid analysis was carried out by spectrophotometer UV/Vis at 480 nm and HPLC using a C18 column with acetonitrile: methanol: 2-propanol (85:10:5) as the mobile phase for isocratic elution. The research result showed that fermentation within 3 days incubation in dark condition and 4 days under blue light was better than 7 days in dark condition, and produced carotenoid 8.13 μg/g spores. Substrate with addition of maltose 2 % w/v and incubation at aerobic condition produced carotenoid 19.913 μg/g spores. Stimulation by co-factor Mg2+ in the ranges concentration below 10 mM increased the total carotenoids up to 24.31 μg/g spores, but decreased when was stimulated higher than 12 mM. Stimulation by antibiotics penicillin and chloramphenicol at concentration of 1 mg/mL increased the β-carotene content. Penicillin influenced more significant to the carotenogenesis of Neurospora intermedia N-1, particularly for β-carotene production up to 17.193 μg/g spores.

To read the full article Peer-reviewed Article PDF image

Author(s): Marlia Singgih Wendi Andriatna Sophi Damayanti and Sri Priatni

Keywords

Neurospora intermedia, carotenogenesis, carotenoid, co-factor Mg2+, antibiotic, Carotenogenesis , fermentation

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords